Iemand wat kennis het, praat nie baie nie. Iemand met insig bly kalm. Serbian language, cirilica, 31 cm, Olimpijske igre, Srpski olimpijski klub, osnovan 23. Februara 1910 10. Februara po starom kalendaru, bio je prva olimpijska organizacija Juznih Slovena i, ispostavilo se, prenosilac olimpijskog suvereniteta svih olimpijskih organizacija drzava koje su se stvarale i nestajale na ovom tlu. Sijne Koninckl. Van Engelants oratie aen de ledenen van de twee Huysen des Parlements ghedaen, tot Oxford den eersten februarij, 1644: item, een missive van den Marquis van Argile ende Sir Will.

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Author contributions: Zykov VA, Tuchina TP and Lebedev DA performed the experiments and wrote the manuscript; Babenko AY, Kuleshova EV and Grineva EN performed the literature review and suggested the study concept; Krylova IB and Bayramov AA developed the experimental design and performed data analysis; Galagudza MM provided scientific consulting, coordinated experimental parts, and edited the manuscript. Supported by Russian Science Foundation, No. Correspondence to: Alina Y Babenko, DSc, MD, PhD, Doctor, Research Scientist, Institute of Endocrinology, Almazov National Medical Research Centre, Akkuratova St., 2, St-Petersburg 197341, Russia. Telephone: +7-9 Fax: +7-8.

METHODS Type 2 diabetes mellitus (T2DM) was induced in male Wistar rats with streptozotocin (65 mg/kg) and verified using an oral glucose tolerance test. Uchebnik teoriya sudebnoj ekspertizi er rossinskaya zinin. After anesthesia, the left coronary artery was occluded for 40 min followed by 80 min reperfusion. Blood glucose level was measured during surgery. Rats were randomized into six groups as follows: (1) control rats; (2) insulin (0.1 U/kg) treated rats prior to ischemia; (3) insulin (0.1 U/kg) treated rats at reperfusion; (4) GLP-1a (140 mg/kg) treated rats prior to ischemia; (5) GLP-1a (140 mg/kg) treated rats at reperfusion; and (6) rats treated with GLP-1a (140 mg/kg) prior to ischemia plus insulin (0.1 U/kg) at reperfusion.

Myocardial area at risk and infarct size was measured planimetrically using Evans blue and triphenyltetrazolium chloride staining, respectively. RESULTS There was no significant difference in the myocardial area at risk among groups.

Insulin treatment before ischemia resulted in a significant increase in infarct size (34.7% ± 3.4% vs 18.6% ± 3.1% in the control rats, P. Core tip: In addition to their glucose-lowering effects, glucagon-like peptide-1 analogs (GLP-1a) were shown to exhibit cardioprotective effects. However, the optimal protocol of GLP-1a administration for infarct size reduction has not been determined yet. Additionally, it is important to investigate the effects of GLP-1a combined with other antidiabetic drugs on myocardial infarct size. Thus, we evaluated the effects of GLP-1a with and without insulin on infarct size in rats with type 2 diabetes mellitus.